DMSO Buffer Protocol

Materials:

Order from AMRESCO:

DMSO: 0231-500ML

EDTA: E177-500ML

Sodium Chloride: X190-5KG

To prepare 2 liters of buffer, mix in the following order on a magnetic plate:

- 1L d'EDTA

- 0.5L de DMSO

- 0.5L de sterile H2O

- Add gradually Sodium Chloride until saturation (~300g) – Mix on a magnetic plate overnight

If all the salt has dissolved overnight, add more the next day until a 1" salt deposit is visible at the bottom of the container. Saturation is very important to ensure DNA preservation

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